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Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping

SYSNO ASEP	0367249
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping
Author(s)	Patzak, J. (CZ) Matoušek, Jaroslav (BC-A)_{RID, ORCID}
Number of authors	2
Source Title	Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134 Roč. 55, č. 4 (2011), s. 761-765
Number of pages	5 s.
Language	eng - English
Country	NL - Netherlands
Keywords	cluster analysis ; dendrogram ; Humulus lupulus ; simple sequence repeat
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA521/08/0740 GA ČR - Czech Science Foundation (CSF)
	QH81052 GA MZe - Ministry of Agriculture (MZe)
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
UT WOS	000295980500026
DOI	10.1007/s10535-011-0183-7
Annotation	The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect the better relationship among species or cultivars than markers previously used. The first set of 30 EST-SSR was developed in hop (Humulus lupulus L.). They represent 25 gene loci with total of 1268 EST sequences. They were used for characterization of 11 hop samples and cross-amplification in Humulus japonicus Sieb. et Zucc. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182 to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram well matched with genealogical and geographical data for hop genotypes.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1