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Translation Reinitiation Relies on the Interaction between eIF3a/TIF32 and Progressively Folded cis-Acting mRNA Elements Preceding Short uORFs
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SYSNO ASEP 0366863 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Translation Reinitiation Relies on the Interaction between eIF3a/TIF32 and Progressively Folded cis-Acting mRNA Elements Preceding Short uORFs Author(s) Munzarová, Vanda (MBU-M)
Pánek, Josef (MBU-M) RID
Gunišová, Stanislava (MBU-M) RID
Dányi, István (MBU-M)
Szamecz, Bela (MBU-M)
Valášek, Leoš (MBU-M) RIDSource Title PLoS Genetics - ISSN 1553-7390
Roč. 7, č. 7 (2011), s. 1-16Number of pages 16 s. Language eng - English Country US - United States Keywords OPEN READING FRAMES ; INITIATION-FACTORS ; IN-VIVO Subject RIV EE - Microbiology, Virology R&D Projects GA303/09/0475 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000293338600006 DOI 10.1371/journal.pgen.1002137 Annotation Reinitiation is a gene-specific translational control mechanism characterized by the ability of some short upstream uORFs to tain post-termination 40S subunits on mRNA. Its efficiency depends on surrounding cis-acting sequences, uORF elongation rates, various initiation factors, and the intercistronic distance. To unravel effects of cis-acting sequences, we investigated previously unconsidered structural properties of one such a cis-enhancer in the mRNA leader of GCN4 using yeast genetics and biochemistry. This leader contains four uORFs but only uORF1, flanked by two transferrable 59 and 39 cisacting sequences, and allows efficient reinitiation. Recently we showed that the 59 cis-acting sequences stimulate reinitiation by interacting with the N-terminal domain (NTD) of the eIF3a/TIF32 subunit of the initiation factor eIF3 to stabilize post-termination 40S subunits on uORF1 to resume scanning downstream Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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