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Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers
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SYSNO ASEP 0366150 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers Author(s) Marhol, Petr (MBU-M) RID
Gažák, Radek (MBU-M) RID, ORCID
Bednář, P. (CZ)
Křen, Vladimír (MBU-M) RID, ORCIDSource Title Journal of Separation Science. - : Wiley - ISSN 1615-9306
Roč. 34, 16-17 (2011), s. 2206-2213Number of pages 8 s. Language eng - English Country DE - Germany Keywords chromolith ; kinetex ; Core-shell Subject RIV CC - Organic Chemistry R&D Projects ME10027 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP207/10/0288 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000295189400031 DOI 10.1002/jssc.201100309 Annotation Two chromatographic narrow-bore columns, a novel 2.6 µm particle-packed KinetexTM C18 core–shell (50x2.1mm id) and monolithic Chromolith® FastGradient RP-18e (50x2mm id), were evaluated for the analysis of diastereoisomers of the flavonolignans silybin and 23-O-acetylsilybin under isocratic conditions. The main advantages of the core–shell column are markedly higher efficiency (hmin=2.8 versus 5.6 for silybin A) and better peak symmetry. The Kinetex column exhibits only a slight change in the height equivalent of the theoretical plate with a higher linear velocity of the mobile phase. The monolithic column shows notably higher selectivity in terms of selectivity factor (1.21 versus 1.12) in the analysis of critical-pair of diastereoisomers (silybin A and silybin B) and enables shorter run duration (approx. twofold) together with lower backpressure Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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