Number of the records: 1

First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach

SYSNO ASEP	0366036
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach
Author(s)	Vitulo, N. (IT) Albiero, A. (IT) Forcato, C. (IT) Šimková, Hana (UEB-Q)_{RID, ORCID} Kubaláková, Marie (UEB-Q)_RID Perrotta, G. (IT) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Cattivelli, L. (IT)
Number of authors	21
Source Title	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 6, č. 10 (2011), s. 1-14
Number of pages	14 s.
Language	eng - English
Country	US - United States
Keywords	wheat chromosome 5A ; Chromosome arm sorting ; DNA amplification
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	ED0007/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000296186900060
DOI	10.1371/journal.pone.0026421
Annotation	Wheat is one of the world’s most important crops and is characterized by a large polyploid genome. One way to reduce genome complexity is to isolate single chromosomes using flow cytometry. Low coverage DNA sequencing can provide a snapshot of individual chromosomes, allowing a fast characterization of their main features and comparison with other genomes. We used massively parallel 454 pyrosequencing to obtain a 2x coverage of wheat chromosome 5A. The resulting sequence assembly was used to identify TEs, genes and miRNAs, as well as to infer a virtual gene order based on the synteny with other grass genomes. Repetitive elements account for more than 75% of the genome. Gene content was estimated considering non-redundant reads showing at least one match to ESTs or proteins. The results indicate that the coding fraction represents 1.08% and 1.3% of the short and long arm respectively, projecting the number of genes of the whole chromosome to approximately 5,000.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1