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Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues

SYSNO ASEP	0365995
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues
Author(s)	Long, Shaojun (BC-A) Changmai, Piya (BC-A) Tsaousis, A.D. (CA) Skalický, Tomáš (BC-A) Verner, Zdeněk (BC-A) Wen, Yan-Zi (BC-A) Roger, A. J. (CA) Lukeš, Julius (BC-A)_{RID, ORCID}
Source Title	Molecular Microbiology - ISSN 0950-382X Roč. 81, č. 6 (2011), 1403-1418
Number of pages	16 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	IRON-SULFUR CLUSTER ; ESCHERICHIA-COLI ; ASSEMBLY PROTEIN ; SACCHAROMYCES-CEREVISIAE ; AZOTOBACTER-VINELANDII ; CYSTEINE DESULFURASE ; CRYSTAL-STRUCTURE ; BINDING ACTIVITY ; GENE-CLUSTER
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/09/1667 GA ČR - Czech Science Foundation (CSF)
	LC07032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	2B06129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000295087000001
DOI	10.1111/j.1365-2958.2011.07769.x
Annotation	IscA/Isa proteins function as alternative scaffolds for the assembly of Fe-S clusters and/or provide iron for their assembly in prokaryotes and eukaryotes. We have studied their function in Trypanosoma brucei, where the requirement for both of them to sustain cell growth depends on the life cycle stage. Reactive oxygen species but not iron accumulate in the procyclic mitochondrion upon ablation of the TbIsa proteins, but their depletion does not influence the assembly of Fe-S clusters in cytosolic proteins. In the bloodstream form, which has a downregulated mitochondrion, the TbIsa proteins are non-essential. The Isa2 orthologue of the anaerobic protist Blastocystis partially rescued the growth and enzymatic activities of TbIsa1/2 knock-down. Rescues with human Isa orthologues partially recovered the activities of aconitase and fumarase. These results show that the Isa1 and Isa2 proteins of diverse eukaryotes have overlapping functions.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1