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Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes

SYSNO ASEP	0365945
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes
Author(s)	Zatloukalová, Pavlína (UEB-Q) Hřibová, Eva (UEB-Q)_{RID, ORCID} Kubaláková, Marie (UEB-Q)_RID Suchánková, Pavla (UEB-Q)_RID Šimková, Hana (UEB-Q)_{RID, ORCID} Adoración, C. (ES) Kahl, G. (DE) Millán, T. (ES) Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	Chromosome Research. - : Springer - ISSN 0967-3849 Roč. 19, č. 6 (2011), s. 729-739
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	BAC-FISH ; Chromosome isolation ; Flow cytometric sorting
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000300089200003
DOI	10.1007/s10577-011-9235-2
Annotation	Various genetic linkage maps have been established and markers linked to resistance genes been identified in cultivated chickpea. However, until now, only one linkage group (LG) has been assigned to a specific chromosome. In the present work, mitotic chromosomes were sorted using flow cytometry and used as template for PCR with primers designed for genomic regions flanking microsatellites. These primers amplify sequence-tagged microsatellite site markers. To establish a set of preferably diagnostic cytogenetic markers, the genomic distribution of various probes was verified using FISH. Moreover, a partial genomic bacterial artificial chromosome (BAC) library was constructed and putative single/low-copy BAC clones weremapped cytogenetically. As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1