Number of the records: 1

Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS

SYSNO ASEP	0365378
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS
Author(s)	Berkman, O. J. (AT) Skarshewski, A. (AU) Lorenc, M. T. (AU) Kubaláková, Marie (UEB-Q)_RID Šimková, Hana (UEB-Q)_{RID, ORCID} Batley, J. (AU) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Edwards, D. (AU)
Number of authors	17
Source Title	Plant Biotechnology Journal. - : Wiley - ISSN 1467-7644 Roč. 9, č. 7 (2011), s. 768-775
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	wheat genome sequence ; chromosome 7 ; genome assembly
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA521/07/1573 GA ČR - Czech Science Foundation (CSF)
	ED0007/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000293659700006
DOI	10.1111/j.1467-7652.2010.00587.x
Annotation	The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in size and consist predominantly of repetitive elements, making the sequencing and assembly of this genome a major challenge. We have reduced genome sequence complexity by isolating chromosome arm 7DS and applied second-generation technology and appropriate algorithmic analysis to sequence and assemble low copy and genic regions of this chromosome arm. The assembly represents approximately 40% of the chromosome arm and all known 7DS genes. Comparison of the 7DS assembly with the sequenced genomes of rice (Oryza sativa) and Brachypodium distachyon identified large regions of conservation. The syntenic relationship between wheat, B. distachyon and O. sativa, along with available genetic mapping data, has been used to produce an annotated draft 7DS syntenic build, which is publicly available at http://www.wheatgenome.info.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1