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The ITS1-5.8S-ITS2 Sequence Region in the Musaceae: Structure, Diversity and Use in Molecular Phylogeny
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SYSNO ASEP 0365197 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The ITS1-5.8S-ITS2 Sequence Region in the Musaceae: Structure, Diversity and Use in Molecular Phylogeny Author(s) Hřibová, Eva (UEB-Q) RID, ORCID
Čížková, Jana (UEB-Q) RID, ORCID
Christelová, Pavla (UEB-Q) RID, ORCID
Taudien, S. (DE)
De Langhe, E. (BE)
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 3 (2011), e17863-e17863Number of pages 11 s. Language eng - English Country US - United States Keywords INTERNAL TRANSCRIBED SPACER ; NUCLEAR RIBOSOMAL DNA ; RNA SECONDARY STRUCTURE Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA600380703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KJB500380901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000288809100011 DOI 10.1371/journal.pone.0017863 Annotation Genes coding for 45S ribosomal RNA are organized in tandem arrays of up to several thousand copies and contain 18S, 5.8S and 26S rRNA units separated by internal transcribed spacers ITS1 and ITS2. While the rRNA units are evolutionary conserved, ITS show high level of interspecific divergence and have been used frequently in genetic diversity and phylogenetic studies. In this work we report on the structure and diversity of the ITS region in 87 representatives of the family Musaceae. We provide the first detailed information on ITS sequence diversity in the genus Musa and describe the presence of more than one type of ITS sequence within individual species. Both Sanger sequencing of amplified ITS regions and whole genome 454 sequencing lead to similar phylogenetic inferences. We show that it is necessary to identify putative pseudogenic ITS sequences, which may have negative effect on phylogenetic reconstruction at lower taxonomic levels. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2012
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