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Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells
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SYSNO ASEP 0365189 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells Author(s) Szabo, Pavol (UMG-J)
Kolář, Michal (UMG-J) RID, ORCID
Dvořánková, B. (CZ)
Lacina, L. (CZ)
Štork, J. (CZ)
Vlček, Čestmír (UMG-J) RID
Strnad, Hynek (UMG-J) RID
Tvrdek, M. (CZ)
Smetana, K. (CZ)Source Title Biology of the Cell. - : Wiley - ISSN 0248-4900
Roč. 103, č. 5 (2011), s. 233-248Number of pages 16 s. Language eng - English Country FR - France Keywords basal cell carcinoma (BCC) ; stem cell ; tumour stroma Subject RIV EB - Genetics ; Molecular Biology R&D Projects 2B06106 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000292430900003 DOI 10.1042/BC20100113 Annotation Multipotent mesenchymal stem cells can participate in the formation of a microenvironment stimulating the aggressive behaviour of cancer cells. Cells exhibiting pluripotent embryonic stem cell markers (Nanog and Oct4) have been observed in many tumours. Here, we investigate the stem cell supporting role of cancer-associated fibroblasts (CAF). We test the influence of CAF isolated from basal cell carcinoma on mouse 3T3 fibroblasts, focusing on the expression of stem cell markers and plasticity in vitro. We demonstrate the biological activity of CAF by influencing the 3T3 fibroblasts to express markers such as Oct4, Nanog and Sox2 and to show differentiation potential similar to mesenchymal stem cells. The role of growth factors such as IGF2, FGF7, LEP, NGF and TGFβ, produced by CAF, is established to participate in their activity. Our observations indicate the pro-stem cell activity of CAF and underline the role of epithelial–mesenchymal interaction in tumour biology. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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