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Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism
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SYSNO ASEP 0365173 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism Author(s) Teixeira, A.R.L. (BR)
Gomes, C. (BR)
Nitz, N. (BR)
Sousa, A.O. (BR)
Alvez, R.M. (BR)
Guimaro, M.C. (BR)
Cordeiro, C. (BR)
Bernal, F.M. (BR)
Rosa, A.C. (BR)
Hejnar, Jiří (UMG-J) RID
Leonardecz, E. (BR)
Hecht, M.M. (BR)Source Title PLoS Neglected Tropical Diseases. - : Public Library of Science - ISSN 1935-2735
Roč. 5, č. 3 (2011), e1000Number of pages 18 s. Publication form http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001000 - http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001000 Language eng - English Country US - United States Keywords Chagas disease ; Trypanosoma cruzi ; kDNA minicircles ; inbred chicken Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000288940800030 DOI 10.1371/journal.pntd.0001000 Annotation Destructive lesions in the heart are a substantial part of Chagas disease pathogenesis. To understand the origin of the heart disease we used a chicken model system in which infection with the pathogen, Trypanosoma cruzi, can be initiated in the egg, but parasite persistence is precluded. In ovo infection generated parasite-free chicks that retained only the mitochondrial kinetoplast DNA minicircle (kDNA) in their genome. Crossbreeding showed that kDNA was transferred vertically to chicken progeny. kDNA integration in coding regions of dystrophin and other genes was shown by cloning the insert together with adjacent chicken sequences. The pathology of kDNA-mutated chickens included heart failure, inflammatory cardiomegaly, and immune attack of heart fibres carried out by CD45(+), CD8γδ(+), and CD8α lymphocytes. We suggest that genetic alterations resulting from kDNA integration lead to autoimmune-mediated destruction of heart tissue in the absence of T. cruzi parasites. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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