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Interaction of late apoptotic and necrotic cells with vitronectin
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SYSNO ASEP 0365171 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Interaction of late apoptotic and necrotic cells with vitronectin Author(s) Štěpánek, Ondřej (UMG-J) RID, ORCID
Brdička, Tomáš (UMG-J) RID
Angelisová, Pavla (UMG-J) RID
Horváth, Ondřej (UMG-J)
Špička, Jiří (UMG-J)
Stockbauer, P. (CZ)
Man, Petr (MBU-M) RID, ORCID
Hořejší, Václav (UMG-J) RIDSource Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 5 (2011), e19243Number of pages 12 s. Language eng - English Country US - United States Keywords vitronectin ; apoptosis ; necrosis Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) AV0Z50520514 - UMG-J (2005-2011) UT WOS 000290224800019 DOI 10.1371/journal.pone.0019243 Annotation Vitronectin (Vn) is an abundant plasma glycoprotein identified also as a part of extracellular matrix. Vn is substantially enriched at sites of injured, fibrosing, inflamed, and tumor tissues where it is believed to be involved in wound healing and tissue remodeling. Little is known about the mechanism of Vn localization into the damaged tissues. 2E12 antibody has been described to bind a subset of late apoptotic cells. The antigen recognized by 2E12 antibody was identified as Vn. Vn binds to the late apoptotic and necrotic cells in cell cultures in vitro as well as in murine thymus and spleen in vivo. Confocal microscopy revealed that Vn binds to an intracellular cytoplasmic structure after the membrane rupture. Vitronectin could serve as a marker of membrane disruption in necrosis and apoptosis for flow cytometry analysis. Vn binding to dead cells may represent one of the mechanisms of Vn incorporation into the injured tissues. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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