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Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies

SYSNO ASEP	0364996
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection: synchrotron radiation SAXS and cryo-TEM studies
Author(s)	Angelov, Borislav (UMCH-V)_RID Angelova, A. (FR) Filippov, Sergey K. (UMCH-V)_{RID, ORCID, SAI} Karlsson, G. (SE) Terrill, N. (GB) Lesieur, S. (FR) Štěpánek, Petr (UMCH-V)_{RID, ORCID}
Source Title	Soft Matter - ISSN 1744-683X Roč. 7, č. 20 (2011), s. 9714-9720
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	DNA-lipid-surfactant complexes ; CryoTEM ; small-angle X-ray scattering
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	GA202/09/2078 GA ČR - Czech Science Foundation (CSF)
	GAP208/10/1600 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
UT WOS	000295582000022
DOI	10.1039/C1SM06447A
Annotation	Towards potential treatment of neurodegenerative disorders by nanomedicine gene therapy, a nonviral gene delivery system is created by complexation of novel PEGylated cationic lipid assemblies and a plasmid DNA encoding for the protein brain-derived neurotrophic factor (BDNF). The generated monoolein-based liquid crystalline nanocarriers for BDNF gene transfer are characterized by means of small-angle X-ray scattering (SAXS), cryogenic transmission electron microscopy (cryo-TEM) techniques, and turbidity measurements. Morphologically variable populations of sterically stabilized, PEGylated multicompartment nanoobjects, with confined supercoiled DNA, are established by cryo-TEM imaging. Synchrotron radiation SAXS measurements, performed with diluted nanoparticulate dispersions, evidence the inner multilamelar structure of pear-type vesicles and flexible onion-like objects functionalized by pBDNF. Obtained structural data are of fundamental interest for improvement of brain-targeted gene delivery.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2012

Number of the records: 1