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The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
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SYSNO ASEP 0364917 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions Author(s) Hansel, R. (DE)
Loehr, F. (DE)
Foldynová-Trantírková, Silvie (BC-A) RID
Bamberg, E. (DE)
Trantírek, L. (NL)
Doetsch, V. (DE)Source Title Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 39, č. 13 (2011), 5768-5775Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords MOLECULAR CROWDING CONDITIONS ; INTRAMOLECULAR G-QUADRUPLEX ; CELL NMR-SPECTROSCOPY ; C-MYC PROMOTER ; K+ SOLUTION ; DNA ; STABILITY ; PROTEIN ; POLYMORPHISM ; HYDRATION Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000293020000044 DOI 10.1093/nar/gkr174 Annotation G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the conformational behavior of the telomeric repeats in X. laevis egg extract or in Ficoll is notably different from that observed in the presence of PEG. While the behavior of the telomeric repeat in X. laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2012
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