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Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition

SYSNO ASEP	0364524
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition
Author(s)	Vodičková Kepková, Kateřina (UZFG-Y)_ORCID Vodička, Petr (UZFG-Y)_ORCID Toralová, Tereza (UZFG-Y)_ORCID Lopatářová, M. (CZ) Čech, S. (CZ) Doležel, R. (CZ) Havlíček, V. (CZ) Besenfelder, U. (AT) Kuzmany, H. (AT) Sirard, M. A. (CA) Laurinčik, J. (SK) Kaňka, Jiří (UZFG-Y)_{RID, ORCID}
Number of authors	12
Source Title	Theriogenelogy. - : Elsevier - ISSN 0093-691X Roč. 75, č. 9 (2011), s. 1582-1595
Number of pages	14 s.
Language	eng - English
Country	US - United States
Keywords	OOCYTE MATURATION MEDIA ; MESSENGER-RNA LEVELS ; EARLY EMBRYOGENESIS
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	1M0538 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA523/09/1035 GA ČR - Czech Science Foundation (CSF)
	GD204/09/H084 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000290925500002
DOI	10.1016/j.theriogenology.2010.12.019
Annotation	Pre-implantation embryos derived by in vitro fertilization differ in their developmental potential from embryos obtained in vivo. In order to characterize changes in gene expression profiles caused by in vitro culture environment, we employed microarray constructed from bovine oocyte and preimplantation embryo-specific cDNAs (BlueChip, Universite Laval, Quebec). The analysis revealed changes in the level of 134 transcripts between in vitro derived (cultured in COOK BVC/BVB media) and in vivo derived 4-cell stage embryos and 97 transcripts were differentially expressed between 8-cell stage in vitro and in vivo embryos. The expression profiles of 7 selected transcripts (BUB3, CUL1, FBL, NOLC1, PCAF, GABPA and CNOT4) were studied in detail. We have identified a switch from Cullin 1-like transcript variant 1 to Cullin 1 transcript variant 3 (UniGene IDs BT.36789 and BT.6490, respectively) expressions around the time of bovine major gene activation (8-cell stage).
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2012

Number of the records: 1