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Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization

SYSNO ASEP	0364474
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization
Author(s)	Zalésna, A. (PL) Choleva, Lukáš (UZFG-Y)_{RID, ORCID} Ogielska, M. (PL) Rábová, Marie (UZFG-Y)_RID Marec, František (BC-A)_{RID, ORCID} Ráb, Petr (UZFG-Y)_{RID, ORCID}
Number of authors	6
Source Title	Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581 Roč. 134, č. 3 (2011), s. 206-212
Number of pages	7 s.
Language	eng - English
Country	CH - Switzerland
Keywords	Amphibia ; Chromosomes ; Genomic in situ hybridization (GISH)
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LC06073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA523/09/2106 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000292469000006
DOI	10.1159/000327716
Annotation	The Western Palearctic water frogs Pelophylax ridibundus and P. lessonae were identified as parental (sexual) species and P. esculentus as their interspecific, hybridogenetically reproducing hybrid with hemiclonal heredity. We used genomic in situ hybridization (GISH) to identify parental chromosomes of P. lessonae and P. ridibundus in diploid P. esculentus karyotypes (2n = 26). GISH probes were made by fluorochrome labeling of total genomic DNA extracted from the sexual progenitors. The labeled probe from one species was hybridized to chromosomes of P. esculentus in the presence of excess of unlabeled genomic DNA from the other species. Thus, the P. lessonae probe was blocked by P. ridibundus unlabeled DNA, and vice versa. We successfully discriminated each of the 13 respective parental chromosomes in metaphase complements of the hybrids according to species-specific hybridization signals.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2012

Number of the records: 1