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Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster

SYSNO ASEP	0364369
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster
Author(s)	Košťál, Vladimír (BC-A)_{RID, ORCID} Korbelová, J. (CZ) Rozsypal, J. (CZ) Zahradníčková, Helena (BC-A)_{RID, ORCID} Cimlová, Jana (BC-A)_RID Tomčala, Aleš (BC-A)_RID Šimek, Petr (BC-A)_{RID, ORCID}
Number of authors	7
Source Title	PLoS ONE. - : Public Library of Science - ISSN 1932-6203 Roč. 6, č. 9 (2011), e25025
Number of pages	10 s.
Language	eng - English
Country	US - United States
Keywords	Drosophila melanogaster
Subject RIV	ED - Physiology
R&D Projects	GA206/07/0269 GA ČR - Czech Science Foundation (CSF)
	GA203/09/2014 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000295262100032
DOI	10.1371/journal.pone.0025025
Annotation	We found that long-term cold acclimation considerably improved cold tolerance in fully grown third-instar larvae of D. melanogaster. A comparison of the larvae acclimated at constant 25°C with those acclimated at constant 15°C followed by constant 6°C for 2 d (15°C→6°C) showed that long-term cold acclimation extended the lethal time for 50% of the population (Lt50) during exposure to constant 0°C as much as 630-fold (from 0.137 h to 86.658 h). Such marked physiological plasticity in Lt50 (in contrast to LLT) suggested that chronic indirect chilling injury at 0°C differs from that caused by cold shock. Long-term cold acclimation modified the metabolomic profiles of the larvae. Accumulations of proline (up to 17.7 mM) and trehalose (up to 36.5 mM) were the two most prominent responses. In addition, restructuring of the glycerophospholipid composition of biological membranes was observed.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1