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Single-cell gene-expression profiling and its potential diagnostic applications
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SYSNO ASEP 0364328 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Single-cell gene-expression profiling and its potential diagnostic applications Author(s) Stahlberg, A. (SE)
Kubista, Mikael (BTO-N) RID
Aman, P. (SE)Source Title Expert Review of Molecular Diagnostics . - : Taylor & Francis - ISSN 1473-7159
Roč. 11, č. 7 (2011), s. 735-740Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords gene-expression profiling ; RT-qPCR ; single-cell gene-expression profiling Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP303/10/1338 GA ČR - Czech Science Foundation (CSF) GA301/09/1752 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000294848400014 DOI 10.1586/ERM.11.60 Annotation Gene-expression profiling has been successfully applied in various diagnostic applications, but its full capacity is yet to be realized. Samples are generally prepared from a mixture of different cells that are present in unknown proportions. Cells are, in many aspects, unique in their characteristics and this heterogeneity confounds the expression profile. The development of new and robust techniques to measure gene expression in single cells opens new avenues in molecular medicine. Today, gene-expression profiles of individual cells can be measured with high precision and accuracy, identifying different cell types as well as revealing heterogeneity among cells of the same kind. Here, we review practical aspects of single-cell gene-expression profiling using reverse transcription quantitative real-time PCR and its potential use in diagnostics Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2012
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