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A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae
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SYSNO ASEP 0364285 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A Genomewide Screen for Tolerance to Cationic Drugs Reveals Genes Important for Potassium Homeostasis in Saccharomyces cerevisiae Author(s) Barreto, L. (ES)
Canadell, D. (ES)
Petrezsélyová, Silvia (FGU-C)
Navarrete, C. (ES)
Marešová, Lydie (FGU-C)
Peréz-Valle, J. (ES)
Herrera, R. (ES)
Olier, I. (ES)
Giraldo, J. (ES)
Sychrová, Hana (FGU-C) RID, ORCID
Yenush, L. (ES)
Ramos, J. (ES)
Ariňo, J. (ES)Source Title Eukaryotic Cell - ISSN 1535-9778
Roč. 10, č. 9 (2011), s. 1241-1250Number of pages 10 s. Language eng - English Country US - United States Keywords Potassium homeostasis ; Saccharomyces cerevisiae ; genomewide screen Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC531 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA500110801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000294453000010 DOI 10.1128/EC.05029-11 Annotation Here, we describe a genome-wide screen for mutants that present altered tolerance to hygromycin B, spermine and tetramethylammonium. Two hundred twenty six mutant strains displayed altered tolerance to all three drugs (202 hypersensitive and 24 hypertolerant) and more than 50% presented a strong or moderate growth defect at limiting potassium (1 mM). Functional groups such as protein kinases and phosphatases, intracellular trafficking, transcription or cell cycle and DNA processing were enriched. Essentially, our screen has identified a substantial number of genes which were not previously described to play a direct or indirect role in potassium homeostasis. A subset of 27 representative mutants were selected and subjected to diverse biochemical tests that, in some cases, allow us to postulate the basis for the observed phenotypes Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2012
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