Number of the records: 1

Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases

SYSNO ASEP	0364215
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases
Author(s)	Kielkowski, Pavel (UOCHB-X)_RID Macíčková-Cahová, Hana (UOCHB-X)_{RID, ORCID} Pohl, Radek (UOCHB-X)_{RID, ORCID} Hocek, Michal (UOCHB-X)_{RID, ORCID}
Number of authors	4
Source Title	Angewandte Chemie - International Edition. - : Wiley - ISSN 1433-7851 Roč. 50, č. 37 (2011), s. 8727-8730
Number of pages	4 s.
Language	eng - English
Country	DE - Germany
Keywords	alkynes ; DNA ; protecting groups ; nucleotides ; restriction endonucleases
Subject RIV	CC - Organic Chemistry
R&D Projects	GA203/09/0317 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000295261200055
DOI	10.1002/anie.201102898
Annotation	The first transient chemical protection of DNA against RE cleavage was developed. (Triethylsilyl)ethynyl-modified 7-deaza-dATP is readily incorporated to DNA by primer extension or PCR using KOD XL polymerase. The silylethynyl-protected DNA resists the cleavage by restriction endonucleases (REs). After treatment with NH3, the deprotected DNA is fully cleavable by the REs.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2012

Number of the records: 1