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BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes

SYSNO ASEP	0364122
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes
Author(s)	Šimková, Hana (UEB-Q)_{RID, ORCID} Šafář, Jan (UEB-Q)_{RID, ORCID} Kubaláková, Marie (UEB-Q)_RID Suchánková, Pavla (UEB-Q)_RID Čihalíková, Jarmila (UEB-Q)_RID Robert-Quatre, Heda (UEB-Q) Azhaguvel, P. (US) Weng, Y. Q. (US) Peng, J. (US) Lapitan, N. L. V. (US) Ma, Y. Q. (US) You, F. M. (US) Luo, M. C. (US) Bartoš, Jan (UEB-Q)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	Journal of Biomedicine and Biotechnology - ISSN 1110-7243 -, č. 302543 (2011), s. 1-11
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	TRITICUM-AESTIVUM L. ; HEXAPLOID WHEAT ; BREAD WHEAT
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA521/07/1573 GA ČR - Czech Science Foundation (CSF)
	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000286232600001
DOI	10.1155/2011/302543
Annotation	Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than milion clones, which makes thein screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistence gene Gb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1