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Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

SYSNO ASEP	0364101
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production
Author(s)	Plchová, Helena (UEB-Q)_{RID, ORCID} Moravec, Tomáš (UEB-Q)_{RID, ORCID} Dědič, P. (CZ) Čeřovská, Noemi (UEB-Q)_{RID, ORCID}
Source Title	Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785 Roč. 159, č. 2 (2011), s. 130-132
Number of pages	3 s.
Language	eng - English
Country	DE - Germany
Keywords	Potato leafroll virus ; recombinant viral antigen ; antibody production
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	QH71123 GA MZe - Ministry of Agriculture (MZe)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000285968400012
DOI	10.1111/j.1439-0434.2010.01740.x
Annotation	Vector pMPM-A4 Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp(43-616) and RdRp(304-537) were chosen for expression. A high level of CP and RdRp(304-537) was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1