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The use of oligoperoxide-coated magnetic nanoparticles to label stem cells

SYSNO ASEP	0363241
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The use of oligoperoxide-coated magnetic nanoparticles to label stem cells
Author(s)	Šponarová, Daniela (UMCH-V) Horák, Daniel (UMCH-V)_{RID, ORCID} Trchová, Miroslava (UMCH-V)_{RID, ORCID} Jendelová, Pavla (UEM-P)_{RID, ORCID} Herynek, V. (CZ) Mitina, N. (UA) Zaichenko, A. (UA) Stoika, R. (UA) Lesný, Petr (UEM-P) Syková, Eva (UEM-P)_RID
Source Title	Journal of Biomedical Nanotechnology. - : American Scientific Publishers - ISSN 1550-7033 Roč. 7, č. 3 (2011), s. 384-394
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	magnetic ; nanoparticles ; stem cells
Subject RIV	FH - Neurology
R&D Projects	GA203/09/1242 GA ČR - Czech Science Foundation (CSF)
	GAP503/10/0664 GA ČR - Czech Science Foundation (CSF)
	1M0538 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KAN201110651 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	KAN401220801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
	AV0Z50390703 - UEM-P (2007-2013)
UT WOS	000292858900008
DOI	10.1166/jbn.2011.1289
Annotation	Iron oxide nanoparticles obtained by the coprecipitation of Fe(II) and Fe(III) salts and oxidation were coated with a novel poly(vinyl acetate-co-5-tert-(butylperoxy)-5-methylhex-1-en-3-yne-co-butyl acrylate-co-maleic anhydride) (PVBM) oligomer to ensure colloidal stability. The magnetic nanoparticles were thoroughly characterized by a range of physico-chemical methods, which proved the presence of the coating on the particles. Experiments with rat mesenchymal stem cells (rMSCs) confirmed that PVBM-coated γ-Fe2O3 nanoparticles were not cytotoxic and that the average efficiency of stem cell labeling was good and comparable to that obtained with commercial agents. The cells labeled with PVBM-coated γ-Fe2O3 nanoparticles displayed excellent contrast on magnetic resonance (MR) images. Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers possibility of additional modification by grafting compounds that reduce non-specific protein adsorption.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2012

Number of the records: 1