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Poly(HEMA) brushes emerging as a new platform for direct detection of food pathogen in milk samples
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SYSNO ASEP 0362960 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Poly(HEMA) brushes emerging as a new platform for direct detection of food pathogen in milk samples Author(s) Rodriguez-Emmenegger, Cesar Adolfo (UMCH-V) RID
Avramenko, Oxana (UMCH-V)
Brynda, Eduard (UMCH-V) RID
Škvor, J. (CZ)
Bologna Alles, A. (UY)Source Title Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 26, č. 11 (2011), s. 4545-4551Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords surface plasmon resonance ; food-borne pathogens ; polymer brushes Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GAP503/10/0664 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000293104100046 DOI 10.1016/j.bios.2011.05.021 Annotation The attachment of antibodies onto poly(HEMA) brushes prepared by surface initiated ATRP provided a successful way to fabricate SPR biosensors capable of in-real time detection Cronobacter at concentrations down to 106 cells mL−1 in milk. Non-specific responses to deposition of non-targeted compounds ruled out other biosensors based on poly(MeOEGMA) and poly(HOEGMA) brushes or SAMs of EG2/COEG and EG6 from detection of Cronobacter at low concentrations even if the lowest detection limit of 104 cells mL−1 in PBS was reached by biosensor based on SAM of EG6. Owing to well-controlled character of ATRP it was possible to minimize the thickness of the polymer brushes films so that the fouling could be still suppressed while the capture of antigens by antibodies immobilized on the brush layer could take place close to the gold SPR surface to provide a stronger optical response. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2012
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