Number of the records: 1

Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen

SYSNO ASEP	0362689
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen
Author(s)	Košťál, Vladimír (BC-A)_{RID, ORCID} Zahradníčková, Helena (BC-A)_{RID, ORCID} Šimek, Petr (BC-A)_{RID, ORCID}
Number of authors	3
Source Title	Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424 Roč. 108, č. 32 (2011), s. 13041-13046
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	insect freeze tolerance ; cold hardiness ; glass transition
Subject RIV	ED - Physiology
R&D Projects	GA206/07/0269 GA ČR - Czech Science Foundation (CSF)
	GA203/09/2014 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000293691400023
DOI	10.1073/pnas.1107060108
Annotation	The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196°C) in a fully hydrated state. We examined the associations between the physiological and biochemical parameters of differently acclimated larvae and their freeze tolerance. Profiling of 61 different metabolites identified proline as a prominent compound whose concentration increased from 20 to 147 mM during diapause transition and subsequent cold acclimation. This study provides direct evidence for the essential role of proline in high freeze tolerance. Differential scanning calorimetry analysis suggested that high proline levels, in combination with a relatively low content of osmotically active water and freeze dehydration, increased the propensity of the remaining unfrozen water to undergo a glass-like transition (vitrification) and thus facilitated the prevention of cryoinjury.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1