Number of the records: 1
Low-molecular-weight chitosans: Preparation and characterization
- 1.
SYSNO ASEP 0361593 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Low-molecular-weight chitosans: Preparation and characterization Author(s) Tishchenko, Galina (UMCH-V) RID
Šimůnek, Jiří (UZFG-Y) RID
Brus, Jiří (UMCH-V) RID, ORCID
Netopilík, Miloš (UMCH-V) RID
Pekárek, Michal (UMCH-V) ORCID
Walterová, Zuzana (UMCH-V)
Koppová, Ingrid (UZFG-Y) RID
Lenfeld, Jiří (UMCH-V) RIDSource Title Carbohydrate Polymers. - : Elsevier - ISSN 0144-8617
Roč. 86, č. 2 (2011), s. 1077-1081Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords low-molecular-weight chitosans ; chitooligosaccharides ; oxidative depolymerization Subject RIV ED - Physiology R&D Projects GA525/08/0803 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40500505 - UMCH-V (2005-2011) AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000294187400094 DOI 10.1016/j.carbpol.2011.04.073 Annotation The chitosan depolymerization products prepared by enzymatic hydrolysis, oxidative reaction or microwave irradiation were characterized by FT-IR, MALDI TOF MS, solid state 13C CP/MAS NMR and GPC. Enzymatic hydrolysis resulted in two fractions of low-molecular-weight (LMW) chitosans with Mw 34 kDa (3.9 wt%) and 14.6 kDa (8.5 wt%). In the oxidative reaction at the hydrogen peroxide and acetic acid molar ratio equal to 1.7, two LMW chitosans with Mw 9 and 10 kDa (the deacetylation degree 69% and 94%, respectively) were isolated with the total yield 86 wt%. In microwave irradiation, the Mw of chitosan (175.5 kDa) decreased at most in two times. Two chitosan fractions with Mw 80 and 120 kDa (total yield 70 wt%) were obtained. In all cases, LMW chitosans served as fortifiers for chitosan oligomers, which were retained with the former through multiple hydrogen bonds. Their content in LMW chitosans was roughly 20–30 wt%. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2012
Number of the records: 1