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Effect of pH on the denitrifying enzyme activity in pasture soils in relation to the intrinsic differences in denitrifier communities
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SYSNO ASEP 0361563 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of pH on the denitrifying enzyme activity in pasture soils in relation to the intrinsic differences in denitrifier communities Author(s) Čuhel, Jiří (BC-A)
Šimek, Miloslav (BC-A) RID, ORCIDSource Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 56, č. 3 (2011), s. 230-235Number of pages 6 s. Language eng - English Country CZ - Czech Republic Keywords pH ; denitrifying enzyme activity ; pasture soils Subject RIV EH - Ecology, Behaviour R&D Projects LC06066 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA600660605 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z60660521 - UPB-H, BC-A (2005-2011) UT WOS 000292564200007 DOI 10.1007/s12223-011-0045-x Annotation The effects of pH on denitrifying enzyme activity (DEA) and on the ratio of the denitrification products, N2O and N2, were determined in three pasture soils differing in cattle impact. The linkage between intrinsic differences in the denitrifying communities and pH effects on relative N2O production was also assessed. Soil pH values were adjusted just before DEA determination to obtain soil slurries with a range of pH values. The intrinsic differences in the denitrifier communities were assessed by measuring the kinetic constants of NO3- and N2O reductions. DEA for all three soils was highest at pH 8.4, regardless of native soil pH. Because DEA has typically been measured at native soil pH, our results suggest that DEA might have been underestimated in many previous studies. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2012
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