Number of the records: 1

Quantification of methyl thiocyanate in the headspace of Pseudomonas aeruginosa cultures and in the breath of cystic fibrosis patients by selected ion flow tube mass spectrometry

SYSNO ASEP	0361411
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Quantification of methyl thiocyanate in the headspace of Pseudomonas aeruginosa cultures and in the breath of cystic fibrosis patients by selected ion flow tube mass spectrometry
Author(s)	Shestivska, Violetta (UFCH-W)_{RID, ORCID} Němec, A. (CZ) Dřevínek, P. (CZ) Sovová, Kristýna (UFCH-W)_RID Dryahina, Kseniya (UFCH-W)_{RID, ORCID} Španěl, Patrik (UFCH-W)_{RID, ORCID}
Source Title	Rapid Communications in Mass Spectrometry. - : Wiley - ISSN 0951-4198 Roč. 25, č. 18 (2011), s. 2459-2467
Number of pages	9 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	pseudomonas aeruginosa cultures ; flow tube mass spectrometry ; methyl thiocyanate
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	GA203/09/0256 GA ČR - Czech Science Foundation (CSF)
	GP203/09/P172 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000294267200013
DOI	10.1002/rcm.5146
Annotation	Infection by Pseudomonas aeruginosa (PA) is a major cause of morbidity and mortality in patients with cystic fibrosis (CF). Breath analysis could potentially be a useful diagnostic of such infection, and analyses of volatile organic compounds (VOCs) emitted from PA cultures are an important part of the search for volatile breath markers of PA lung infection. Our pilot experiments using solid-phase microextraction, SPME and gas chromatography/mass spectrometric (GC/MS) analyses of volatile compounds produced by PA strains indicated a clear presence of methyl thiocyanate. This provided a motivation to develop a method for real-time online quantification of this compound by selected ion flow tube mass spectrometry, SIFT-MS.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2012

Number of the records: 1