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Divalent metal ion effect on helix-coil transition of high molecular weight DNA in neutral and alkaline solutions

SYSNO ASEP	0361375
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Divalent metal ion effect on helix-coil transition of high molecular weight DNA in neutral and alkaline solutions
Author(s)	Sorokin, V. A. (UA) Valeev, V. A. (UA) Usenko, E. L. (UA) Andrushchenko, Valery (UOCHB-X)_{RID, ORCID}
Number of authors	4
Source Title	International Journal of Biological Macromolecules. - : Elsevier - ISSN 0141-8130 Roč. 48, č. 2 (2011), s. 369-374
Number of pages	6 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	DNA melting ; alkaline solution ; metal complex ; differential UV spectroscopy ; thermal denaturation
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	GAP208/10/0559 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000288408500022
DOI	10.1016/j.ijbiomac.2010.12.016
Annotation	Effect of Mg2+, Ca2+, Ni2+ and Cd2+ ions on parameters of DNA helix-coil transition in sodium cacodylate (pH 6.5), Tris (pH 8.5) and sodium tetraborate (pH 9.0) buffers have been studied by differential UV-visible spectroscopy and thermal denaturation. Anomalous behavior of the melting temperature Tm and the melting interval .delta.Т in the presence of MgCl2 was observed in Tris, but not in cacodylate or tetraborate buffers. Decrease of the Tm and .delta.Т of DNA in the presence of Ni2+ and Cd2+ was caused by preferential ion interaction with N7 of guanine. This type of interaction was also found for Mg2+ in Tris buffer. The anomalous decrease in the Tm and .delta.Т values was connected to formation of complexes between metal ions and Tris molecules.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2012

Number of the records: 1