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Haplotype analysis of the interleukin-18 gene in Czech patients with allergic disorders

SYSNO ASEP	0360883
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Haplotype analysis of the interleukin-18 gene in Czech patients with allergic disorders
Author(s)	Izakovicova Holla, L. (CZ) Hrdličková, B. (CZ) Schüller, M. (CZ) Bučková, Dana (UZFG-Y) Kindlova, D. (CZ) Izakovic, V. (CZ) Vasku, A. (CZ)
Number of authors	7
Source Title	Human Immunology. - : Elsevier - ISSN 0198-8859 Roč. 71, č. 6 (2010), s. 592-597
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	allergic diseases ; asthma ; IL-18
Subject RIV	EB - Genetics ; Molecular Biology
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000278426100009
DOI	10.1016/j.humimm.2010.03.004
Annotation	The interleukin-18 (IL-18) gene on chromosome 11q22 has been suggested as a susceptibility factor for allergies. To test for a possible role of IL-18 polymorphisms in Czech population, case-control study including 958 subjects (633 allergic patients and 325 healthy controls) was performed. An allele-specific polymerase chain reaction was used to analyze variants at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the promoter region together with the polymerase chain reaction-restriction fragment length polymorphism method for the detection of polymorphism at position -140 C/G (previously -133 C/G, rs360721) in intron 1 of the IL-18 gene. The -1297 C/T (rs360719) polymorphism was genotyped by real-time-polymerase chain reaction, using a predevelopment TaqMan allele discrimination assay. There were no significant differences in distribution of alleles or genotypes in any of four single nucleotide polymorphisms in the IL-18 gene between controls and patients.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2012

Number of the records: 1