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Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms
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SYSNO ASEP 0360805 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms Author(s) Zigo, Michal (BTO-N) RID
Jonáková, Věra (BTO-N) RID
Maňásková-Postlerová, Pavla (BTO-N) ORCIDNumber of authors 3 Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 32, č. 11 (2011), s. 1309-1318Number of pages 10 s. Language eng - English Country DE - Germany Keywords Extraction methods ; Sperm proteins ; Substrate zymography Subject RIV CE - Biochemistry R&D Projects GA303/09/1285 GA ČR - Czech Science Foundation (CSF) NS10009 GA MZd - Ministry of Health (MZ) 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000291555900007 DOI 10.1002/elps.201000558 Annotation Recognition and the binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. Despite longstanding research efforts to identify the sperm proteins that recognize ZP receptors, their precise determination still remains questionable in various mammalian species. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by agents as follows: Triton X-100, Triton X-114, acetic acid, SDS, n-octyl-β-D-glucopyranosid, extraction by freezing-thawing and characterized by 1D, 2D electrophoreses glycoprotein staining and substrate zymographic methods. Results have shown the quantitative and qualitative differences in 1D, 2D protein and glycoprotein profiles depending upon the isolation protocol. Differences were observed between protein profiles of ejaculated and capacitated sperms as well. Zymograms supported the prediction that using various isolation protocols gives various profiles of enzymatically active molecules. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2012
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