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Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase

SYSNO ASEP	0360800
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase
Author(s)	Grulich, Michal (MBU-M)_RID Maršálek, Jaroslav (MBU-M) Kyslík, Pavel (MBU-M)_{RID, ORCID} Štěpánek, Václav (MBU-M)_{RID, ORCID} Kotík, Michael (MBU-M)_{RID, ORCID}
Source Title	Process Biochemistry. - : Elsevier - ISSN 1359-5113 Roč. 46, č. 2 (2011), s. 526-532
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Overexpression ; Fed-batch ; Aqueous two-phase system
Subject RIV	EE - Microbiology, Virology
R&D Projects	GAP207/10/0135 GA ČR - Czech Science Foundation (CSF)
	GAP504/10/0137 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000287274000015
DOI	10.1016/j.procbio.2010.10.004
Annotation	Optimised culture conditions for the recombinant prototrophic strain Escherichia coli RE3(pSEKau2) were established to obtain biomass of epoxide hydrolase (EH). The recombinant enzyme was extracted from crude extracts using an optimised polyethylene glycol-based aqueous two-phase system (ATPS). Eupergit C 250L carrier proved to be the most interesting in terms of partially purified EH immobilization. The immobilized EH exhibited a significantly higher thermal stability compared to the free enzyme. Immobilization did not alter, to a large extent, the enantioselectivity and regioselectivity of the enzyme
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2012

Number of the records: 1