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High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs
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SYSNO ASEP 0360788 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs Author(s) Cantarella, L. (IT)
Gallifuoco, A. (IT)
Malandra, A. (IT)
Martínková, Ludmila (MBU-M) RID, ORCID
Spera, A. (IT)
Cantarella, M. (IT)Source Title Enzyme and Microbial Technology. - : Elsevier - ISSN 0141-0229
Roč. 48, 4-5 (2011), 345-350Number of pages 6 s. Language eng - English Country US - United States Keywords Nitrile hydratase-amidase cascade system ; 3-Cyanopyridine bioconversion ; Nicotinic acid Subject RIV EE - Microbiology, Virology R&D Projects OC09046 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000289930900007 DOI 10.1016/j.enzmictec.2010.12.010 Annotation High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase–amidase enzymatic cascade system of Microbacterium imperiale CBS 498-74. Experiments were carried out in continuously stirred tank UF-membrane bioreactors (CSMRs) arranged in series. This reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences. To this end, the first CSMR, optimized for the properties of the NHase, was operated (i) at low temperature (5◦C), limiting inactivation of the more fragile enzyme, nitrile hydratase, (ii) with a high residence time (24h) to overcome reaction rate limitation Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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