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Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction
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SYSNO ASEP 0360786 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction Author(s) Vejvoda, Vojtěch (MBU-M)
Martínková, Ludmila (MBU-M) RID, ORCID
Veselá, Alicja Barbara (MBU-M) RID
Kaplan, Ondřej (MBU-M)
Lutz-Wahl, S. (DE)
Fischer, L. (DE)
Uhnáková, Bronislava (MBU-M)Source Title Journal of Molecular Catalysis B-Enzymatic. - : Elsevier - ISSN 1381-1177
Roč. 71, 1-2 (2011), s. 51-55Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords Nitrile hydratase ; Rhodococcus erythropolis ; Amidase Subject RIV EE - Microbiology, Virology R&D Projects LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC09046 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000291451000008 DOI 10.1016/j.molcatb.2011.03.008 Annotation The transformation of benzonitrile into benzohydroxamic acid was performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step was catalyzed by either a cell-free extract from Rhodococcus erythropolis A4 (nitrile hydratase) or cell-free extracts from recombinant Escherichia coli strains expressing nitrile hydratases from Raoultella terrigena 77.1 and Klebsiella oxytoca 38.1.2; the biocatalyst in the second step was a cellfree extract from R. erythropolis A4 (amidase). When using the cell-free extract from R. erythropolis A4 in the first step, the hydrolytic amidase activity was suppressed by ammonium ions, which, however, did not inhibit the acyl transfer reaction catalyzed by the same enzyme. Aromatic and aliphatic nitriles were examined as substrates of the recombinant nitrile hydratases by using a colorimetric assay of hydroxamic acids produced in a coupled acyl transfer reaction Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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