Number of the records: 1

Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

SYSNO ASEP	0360698
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10
Author(s)	Kaplan, Ondřej (MBU-M) Bezouška, Karel (MBU-M) Plíhal, Ondřej (MBU-M) Ettrich, Rüdiger (UEK-B)_{RID, ORCID, SAI} Kulik, Natallia (UEK-B)_RID Vaněk, Ondřej (MBU-M) Kavan, Daniel (MBU-M)_{RID, ORCID} Benada, Oldřich (MBU-M)_{ORCID, RID} Malandra, Anna (MBU-M)_ORCID Šveda, Ondřej (MBU-M) Veselá, Alicja Barbara (MBU-M)_RID Rinágelová, Anna (MBU-M) Slámová, Kristýna (MBU-M)_{RID, ORCID} Cantarella, M. (IT) Felsberg, Jürgen (MBU-M) Dušková, Jarmila (UMCH-V)_RID Dohnálek, Jan (UMCH-V)_RID Kotík, Michael (MBU-M)_{RID, ORCID} Křen, Vladimír (MBU-M)_{RID, ORCID} Martínková, Ludmila (MBU-M)_{RID, ORCID}
Source Title	BMC Biotechnology Roč. 11, č. 2 (2011), s. 1-15
Number of pages	15 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	MULTIPLE SEQUENCE ALIGNMENT ; RHODOCOCCUS-RHODOCHROUS J1 ; CRYSTAL-STRUCTURE
Subject RIV	EE - Microbiology, Virology
R&D Projects	IAA500200708 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	OC09046 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GD305/09/H008 GA ČR - Czech Science Foundation (CSF)
	GA310/09/1407 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
	AV0Z40500505 - UMCH-V (2005-2011)
	AV0Z60870520 - UEK-B (2005-2011)
UT WOS	000286401600001
DOI	10.1186/1472-6750-11-2
Annotation	Nitrilases attract increasing attention due to their utility in the mild hydrolysis of nitriles. According to activity and gene screening, filamentous fungi are a rich source of nitrilases distinct in evolution from their widely examined bacterial counterparts. However, fungal nitrilases have been less explored than the bacterial ones. Nitrilases are typically heterogeneous in their quaternary structures, forming short spirals and extended filaments, these features making their structural studies difficult. The nitrilase from Aspergillus niger K10 is highly homologous with proteins deduced from gene sequencing in Aspergillus and Penicillium genera
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2012

Number of the records: 1