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DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay
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SYSNO ASEP 0360452 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay Author(s) Topinka, Jan (UEM-P) RID, ORCID
Rössner ml., Pavel (UEM-P) RID, ORCID
Milcová, Alena (UEM-P)
Schmuczerová, Jana (UEM-P) RID
Švecová, Vlasta (UEM-P) RID
Šrám, Radim (UEM-P) RIDSource Title Toxicology Letters. - : Elsevier - ISSN 0378-4274
Roč. 202, č. 3 (2011), s. 186-192Number of pages 7 s. Language eng - English Country IE - Ireland Keywords air pollution ; genotoxicity ; DNA adducts Subject RIV DN - Health Impact of the Environment Quality R&D Projects 2B08005 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) SP/1B3/8/08 GA MŽP - Ministry of Environment (MŽP) CEZ AV0Z50390512 - UEM-P (2005-2011) UT WOS 000290778600005 EID SCOPUS 79954619520 DOI 10.1016/j.toxlet.2011.02.005 Annotation The genotoxic acitivity of complex mixtures of organic extracts from urban air particles (PM2.5) collected in various localities of the Czech Republic with different levels of air pollution was compared. As markers of genotoxic potential, DNA adduct levels and oxidative DNA damage (8-oxodG levels) induced by EOMs in an acellular assay of calf thymus DNA coupled with 32P-postlabeling (DNA adducts) and ELISA (8-oxodG) were employed. The results indicate that the industrial emissions prevailing in Ostrava-Bartovice represent a substantially higher genotoxic risk that mostly traffic-related emissions in Ostrava-Poruba. B[a]P and c-PAH contents in EOMs are the most important factors relating to their genotoxic potential. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2012
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