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A comprehensive study in triblock copolymer membrane interaction

SYSNO ASEP	0360337
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	A comprehensive study in triblock copolymer membrane interaction
Author(s)	Pembouong, G. (FR) Morellet, N. (FR) Kral, Teresa (UFCH-W)_{RID, ORCID} Hof, Martin (UFCH-W)_{RID, ORCID} Scherman, D. (FR) Bureau, M.-F. (FR) Mignet, N. (FR)
Source Title	Journal of Controlled Release. - : Elsevier - ISSN 0168-3659 Roč. 151, č. 1 (2011), s. 57-64
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	poloxamer ; pluronic ; liposomes
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	IAA400400621 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000291240900009
DOI	10.1016/j.jconrel.2011.01.007
Annotation	Poloxamers are triblock copolymers made of poly(ethylene glycol)-(poly(propylene glycol))-poly(ethylene glycol). They have been shown to enhance gene transfer in the muscle, and co-administration of polymers and DNA appeared to be crucial to obtain this effect. It is questionable then if some interaction occurs between polymers and DNA. Polymer interaction with membranes represents a second crucial point due to the central hydrophobic part of the triblock copolymers. Besides, the question of the polymer spanning or adsorbing to the surface has not been solved by now. We addressed these issues by means of sensitive techniques that allowed working in diluted conditions and gaining in comprehension of gene transfection. By means of simultaneous time-correlated single-photon counting and fluorescence correlation spectroscopy, we have shown that the diffusion time of a single DNA molecule and PicoGreen lifetime was not altered in the presence of the triblock copolymer L64.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2012

Number of the records: 1