Number of the records: 1

The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria

SYSNO ASEP	0360066
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria
Author(s)	Koubová, Anna (BC-A)_{ORCID, RID} Knapp, B.A. (AT) Insam, H. (AT) Pižl, Václav (BC-A)_RID Elhottová, Dana (BC-A)_{RID, ORCID}
Source Title	Acta Societatis Zoologicae Bohemicae - ISSN 1211-376X Roč. 74, - (2010), s. 69-74
Number of pages	6 s.
Action	Central European Workshop on Soil Zoology /10./
Event date	21.04.2009-24.04.2009
VEvent location	České Budějovice
Country	CZ - Czech Republic
Event type	EUR
Language	eng - English
Country	CZ - Czech Republic
Keywords	soil zoology ; soil microbiology ; microbial diversity
Subject RIV	EH - Ecology, Behaviour
R&D Projects	LC06066 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	MEB060814 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	IAA600200704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60660521 - UPB-H, BC-A (2005-2011)
Annotation	The changes that occurred in the diversity of microbes in soil and compost during its passage through the gut of earthworms were evaluated in two epigeic earthworm species Eisenia fetida (Savigny, 1826) and E. andrei (Bouché, 1972), which inhabit forest soil and garden organic compost, respectively. A modified extraction protocol was used to extract DNA from forest soil, compost and fresh earthworm faeces. DNA was amplified in a polymerase chain reaction (PCR). The products were analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprinting. Analysis of archaeal and bacterial DGGE fingerprints revealed that the diversity in both microbial domains increased after passage through the guts of E. andrei, but not E. fetida. Nevertheless, cluster and correspondence analyses revealed that the passage of soil and compost through the guts of E. fetida, E. andrei, respectively, had a significant effect on the composition of the archaea.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1