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Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization
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SYSNO ASEP 0359861 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization Author(s) Hobzová, Radka (UMCH-V) RID, ORCID
Přádný, Martin (UMCH-V) RID
Zhunusbekova, Nazym M. (UMCH-V)
Širc, Jakub (UMCH-V) RID, ORCID
Guryča, Vilém (UMCH-V)
Michálek, Jiří (UMCH-V) RID, ORCIDSource Title E-Polymers. - : EUROPEAN POLYMER FEDERATION - ISSN 1618-7229
-, 043 (2011), s. 1-17Number of pages 17 s. Language eng - English Country US - United States Keywords hydrogel ; poly(2-hydroxyethyl methacrylate) ; surface modification Subject RIV CD - Macromolecular Chemistry R&D Projects 1QS400500558 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000290474600001 DOI 10.1515/epoly.2011.11.1.474 Annotation Synthetic hydrogels are often used in biomedical applications as many of them are compatible with living tissue and moreover they meet the most criteria for artificial tissue properties. To enhance the cell adhesion and proliferation, it is desirable to modify the polymer surface by some bioactive compounds (e.g. saccharides, proteins). In this work, firstly carboxyl-enriched hydrogels were prepared by various methods: by copolymerization of 2 hydroxyethyl methacrylate with methacrylic acid, by hydrolysis or oxidation of poly(2 hydroxyethyl methacrylate). Subsequently, avidin was immobilized to the modified hydrogel surfaces either electrostatically through dissociated carboxylic groups or covalently through carboxylic groups activated by N-hydroxysuccinimide. Preliminary biological tests showed that hydrogels with immobilized avidin provides better adhesion and proliferation of keratinocytes compared to supports without avidin. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2012 Electronic address http://www.e-polymers.org/journal/abstract.cfm?abstract_Id=3616
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