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MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process
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SYSNO ASEP 0359407 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process Author(s) Ammerman, M. L. (US)
Hashimi, Hassan (BC-A) RID, ORCID
Novotná, Lucie (BC-A)
Číčová, Zdeňka (BC-A)
Mcevoy, S. M. (US)
Lukeš, Julius (BC-A) RID, ORCID
Read, L. K. (US)Source Title RNA. - : Cold Spring Harbor Laboratory Press - ISSN 1355-8382
Roč. 17, č. 5 (2011), 865-877Number of pages 13 s. Language eng - English Country US - United States Keywords RNA editing ; trypanosome ; MRB1 complex ; mitochondria ; kinetoplast Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/09/1667 GA ČR - Czech Science Foundation (CSF) 2B06129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC07032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000289617400009 DOI 10.1261/rna.2446311 Annotation In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 "core'' complex containing five to six proteins, including MRB3010. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2012
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