Number of the records: 1

Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization

SYSNO ASEP	0359405
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Hemelipoglycoprotein from the ornate sheep tick, Dermacentor marginatus: structural and functional characterization
Author(s)	Dupejová, J. (CZ) Štěrba, Ján (BC-A)_{RID, ORCID} Vancová, Marie (BC-A)_{RID, ORCID} Grubhoffer, Libor (BC-A)_{RID, ORCID}
Source Title	Parasites & Vectors. - : BioMed Central - ISSN 1756-3305 Roč. 4, January (2011), s. 4
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	ORNITHODOROS PARKERI ; BOOPHILUS MICROPLUS ; MASS-SPECTROMETRY ; CATTLE TICK ; SOFT TICK ; DORIN M ; PROTEINS ; HEMOLYMPH ; VITELLOGENIN ; LIPOPROTEIN
Subject RIV	EA - Cell Biology
R&D Projects	LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KJB600960906 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000286388600001
DOI	10.1186/1756-3305-4-4
Annotation	Results: Hemelipoglycoprotein from sheep tick Dermacentor marginatus plasma was purified in native state by immunoprecipitation and denatured using electroelution from SDS-PAGE separated plasma. The protein (290 kDa) contains two subunits with molecular weights 100 and 95 kDa. It is glycosylated by high-mannose and complex N-glycans HexNAc(2)Hex(9), HexNAc(2)Hex(10), HexNAc(4)Hex(7), and HexNAc(4)Hex(8). The purified protein is able to agglutinate red blood cells and has galactose-and mannose-binding specificity. Feeding does not increase its amounts in male plasma. The hemelipoglycoprotein was detected also in hemocytes, salivary glands, and gut. In salivary glands, the protein was present in both glycosylated and nonglycosylated forms. In hemolymph, it is present in complexes with putative fibrinogen-related proteins. This, together with its carbohydrate-binding activity, suggests its possible involvement in tick innate immunity.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1