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Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation

SYSNO ASEP	0359351
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation
Author(s)	Souček, Karel (BFU-R)_{RID, ORCID} Jirsová, Pavla (BFU-R) Brázdová, Marie (BFU-R)_{RID, ORCID} Hýžďalová, Martina (BFU-R)_{RID, ORCID} Kočí, Lenka (BFU-R) Vydra, D. (CZ) Trojanec, R. (CZ) Pernicová, Zuzana (BFU-R)_RID Lentvorská, L. (CZ) Hajdúch, M. (CZ) Hofmanová, Jiřina (BFU-R)_{RID, ORCID} Kozubík, Alois (BFU-R)_{RID, ORCID}
Number of authors	12
Source Title	Cancer Genetics and Cytogenetics. - : Elsevier - ISSN 0165-4608 Roč. 197, č. 2 (2010), s. 107-116
Number of pages	10 s.
Language	eng - English
Country	US - United States
Keywords	colon epithelial cells ; TP53 ; MYC
Subject RIV	BO - Biophysics
R&D Projects	ME 919 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
UT WOS	000279974200002
DOI	10.1016/j.cancergencyto.2009.11.009
Annotation	The FHC (fetal human cells) cell line has been established from normal fetal colonic mucosa. Detailed characterization of this cell line and mechanism of spontaneously acquired immortality have not been described yet. Therefore, we characterized the FHC cell line in terms of its tumorigenicity, cytogenetics, and TP53 gene mutation analysis. FHC cells displayed capability for anchorage-independent growth in semisolid media in vitro and formed solid tumors after transplantation into SCID (severe combined immunodeficiency) mice. This tumorigenic phenotype was associated with hypotriploidy and chromosome number ranging from 66 to 69. We identified mutation of TP53 gene in codon 273; triplet CGT coding Arg was changed to CAG coding His. Because of their origin from normal fetal colon and their relative resistance to the induction of apoptosis, FHC cells can be considered a valuable experimental model for various studies.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2011

Number of the records: 1