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PIN phosphorylation is sufficient to mediate PIN polarity and direct auxin transport
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SYSNO ASEP 0359312 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title PIN phosphorylation is sufficient to mediate PIN polarity and direct auxin transport Author(s) Zhang, J. (BE)
Nodzynski, T. (BE)
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Rolčík, Jakub (UEB-Q)
Friml, J. (BE)Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 107, č. 2 (2010), s. 918-922Number of pages 5 s. Language eng - English Country US - United States Keywords cell polarity ; auxin distribution ; plant architecture Subject RIV EF - Botanics R&D Projects GA301/08/1649 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000273559300073 DOI 10.1073/pnas.0909460107 Annotation The plant hormone auxin plays a crucial role in regulating plant development and plant architecture. The directional auxin distribution within tissues depends on PIN transporters that are polarly localized on the plasma membrane. The PIN polarity and the resulting auxin flow directionality are mediated by the antagonistic actions of PINOID kinase and protein phosphatase 2A. However, the contribution of the PIN phosphorylation to the polar PIN sorting is still unclear. Here, we identified an evolutionarily conserved phosphorylation site within the central hydrophilic loop of PIN proteins that is important for the apical and basal polar PIN localizations. Inactivation of the phosphorylation site in PIN1(Ala) resulted in a predominantly basal targeting and increased the auxin flow to the root tip. In contrast, the outcome of the phosphomimic PIN1(Asp) manipulation was a constitutive, PINOID-independent apical targeting of PIN1 and an increased auxin flow in the opposite direction. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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