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Function of plant defense secondary metabolite in cytokinin degradation
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SYSNO ASEP 0359310 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Function of plant defense secondary metabolite in cytokinin degradation Author(s) Frébortová, Jitka (UEB-Q) RID, ORCID Number of authors 1 Source Title Plant Signaling & Behavior. - : Taylor & Francis - ISSN 1559-2316
Roč. 5, č. 5 (2010), s. 523-525Number of pages 3 s. Language eng - English Country US - United States Keywords cytokinin dehydrogenace ; 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one ; phenoxyl radiál Subject RIV EF - Botanics R&D Projects GA522/05/0448 GA ČR - Czech Science Foundation (CSF) GA301/08/1649 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation The flavoenzyme cytokinin dehydrogenace (CKX) catalyzes an irreversible deactivation of plant hormones cytokinins through oxidative cleavage of the cytokinin side chain to Šeld adenine or adenosine and an aldehyde. In the catalytic cycle of CKX, the cytokinin-reduced flavin cofactor is reoxidized by a suitable electron acceptor. We have recently demonstrated that the oxidation products of natural hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) function as effective electron acceptors of apoplastic CKX from maize. The stable oxidation product of DIMBOA reacting with peroxidase or laccase was identified as 4-nitrosoresorcinol 1-monomethyl ether (coniferron), which, however, is only a weak electron acceptor of CKX. Further analyses suggested formativ of transient free radicals that were estimated to reoxidize the cytokininreduced flavin cofactor of CKX with the rates comparable to those of flavin reduction. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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