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Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry

SYSNO ASEP	0359308
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry
Author(s)	Tarkowski, Petr (UEB-Q) Václavíková, Kateřina (UEB-Q) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Pertry, I. (BE) Hanuš, Jan (UEB-Q) Whenham, R. (GB) Vereecke, D. (BE) Šebela, M. (CZ) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Number of authors	9
Source Title	Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670 Roč. 680, 1-2 (2010), s. 86-91
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Cytokinins ; Rhodococcus fascians ; High-performance liquid chromatography
Subject RIV	CE - Biochemistry
R&D Projects	GA301/08/1649 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000284385800013
DOI	10.1016/j.aca.2010.09.020
Annotation	A sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete Rhodococcus fascians. The cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (C18 and ion-exchange). The purified samples were further separated and analyzed by HPLC-ESI-MS/MS. This allowed to achieve chromatographic resolution of six highly hydrophobic cytokinin species including 2-methylthio-isopentenyladenine, 2-methylthio-isopentenyladenosine, 2-methylthio-trans-zeatin and 2-methylthio-trans-zeatin riboside and their cis-isomers. Quantification was performed by a standard isotope dilution method using a multiple-reaction monitoring (MRM) mode.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1