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Endogenous brassinosteroids in wheat treated with 24-epibrassinolide
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SYSNO ASEP 0359303 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Endogenous brassinosteroids in wheat treated with 24-epibrassinolide Author(s) Janeczko, A. (PL)
Swaczynová, Jana (UEB-Q)Number of authors 2 Source Title Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 54, č. 3 (2010), s. 477-482Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords brassinolide ; castasterone ; growth response Subject RIV EF - Botanics R&D Projects GA301/08/1649 GA ČR - Czech Science Foundation (CSF) UT WOS 000279079100011 DOI 10.1007/s10535-010-0084-1 Annotation The aim of the study was to examine the effect of exogenous 24-epibrassinolide on its uptake and content of endogenous brassinosteroids in wheat seedlings. 24-Epibrassinolide was applied at two concentrations (0.1 and 2.0 mu M) and in three different methods: by soaking seeds, by drenching and by spraying plants. Brassinosteroids were determined by high-performance liquid chromatography combined with electrospray mass spectrometry. Three important brassinosteroids, 24-epibrassinolide, brassinolide and castasterone, were detected in the wheat leaves, but their contents varied with leaf insertion and plant age. Increased 24-epibrassinolide content in the leaf tissue was found when this hormone was applied by soaking or drenching. Additionally the seed treatment influenced brassinosteroid balance in seedlings. The growth response of wheat seedlings treated with 24-epibrassinolide has been also investigated. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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