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Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells
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SYSNO ASEP 0359295 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells Author(s) Koledová, Z. (CZ)
Rašková-Kafková, L. (CZ)
Calábková, L. (CZ)
Kryštof, Vladimír (UEB-Q) RID, ORCID
Doležel, P. (CZ)
Divoký, V. (CZ)Number of authors 6 Source Title Stem Cells and Development. - : Mary Ann Liebert - ISSN 1547-3287
Roč. 19, č. 2 (2010), s. 181-193Number of pages 13 s. Language eng - English Country US - United States Keywords embryonic stem cells ; cell cycle ; G1 phase Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA301/08/1649 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000274929400005 DOI 10.1089/scd.2009.0065 Annotation Embryonic stem cells (ESCs) proliferate rapidly and have a unique cell-cycle structure with a very short G1 phase. Previous reports suggested that the rapid G1 phase progression of ESCs might be underpinned by high and precocious Cdk2 activity and that Cdk2 activity might be crucial for both cell-cycle regulation and cell-fate decisions in human ESCs. However, the actual role of Cdk2 in cell-cycle progression of mouse ESCs (mESCs) has not been elucidated. In this study, we investigated the effects of down-regulation of Cdk2 activity by olomoucine II in 2 mESC lines. Olomoucine II treatment signifi cantly increased the G1 phase cell numbers, decreased the S phase cell numbers, and inhibited DNA replication in mESCs. In nocodazole-synchronized mESCs, we show that specifi c down-regulation of Cdk2 activity prolongs G1 phase progression. In addition, down-regulation of Cdk2 activity in mESCs established a somatic cell-like cell cycle and induced expression of differentiation markers. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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