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A seed coat bedding assay shows that RGL2-dependent release of abscisic acid by the endosperm controls embryo growth in Arabidopsis dormant seeds
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SYSNO ASEP 0359294 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A seed coat bedding assay shows that RGL2-dependent release of abscisic acid by the endosperm controls embryo growth in Arabidopsis dormant seeds Author(s) Lee, K. P. (CH)
Piskurewicz, U. (CH)
Turečková, Veronika (UEB-Q) RID, ORCID
Strnad, Miroslav (UEB-Q) RID, ORCID
Lopez-Molina, L. (CH)Number of authors 5 Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 107, č. 44 (2010), s. 19108-19113Number of pages 6 s. Language eng - English Country US - United States Keywords gibberellins ; seed dormancy ; DELLA Subject RIV EF - Botanics R&D Projects GA301/08/1649 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000283749000067 DOI 10.1073/pnas.1012896107 Annotation Seed dormancy is an ecologically important adaptive trait in plants whereby germination is repressed even under favorable germination conditions such as imbibition with water. In Arabidopsis and most plant species, dormancy absolutely requires an unidentified seed coat germination-repressive activity and constitutively higher abscisic acid (ABA) levels upon seed imbibition. The mechanisms underlying these processes and their possible relationship are incompletely understood. We developed a "seed coat bedding" assay monitoring the growth of dissected embryos cultured on a layer of seed coats, allowing combinatorial experiments using dormant, nondormant, and various genetically modified seed coat and embryonic materials. This assay, combined with direct ABA measurements, revealed that, upon imbibition, dormant coats, unlike nondormant coats, actively produce and release ABA to repress embryo germination, whatever the embryo origin. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
Number of the records: 1