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An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins

SYSNO ASEP	0359292
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins
Author(s)	Tarkowski, Petr (UEB-Q) Floková, K. (CZ) Václavíková, Kateřina (UEB-Q) Jaworek, P. (CZ) Raus, M. (CZ) Nordström, A. (SE) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Doležal, Karel (UEB-Q)_{RID, ORCID} Šebela, M. (CZ) Frébortová, Jitka (UEB-Q)_{RID, ORCID}
Number of authors	10
Source Title	Molecules. - : MDPI Roč. 15, č. 12 (2010), s. 9214-9229
Number of pages	16 s.
Language	eng - English
Country	CH - Switzerland
Keywords	cytokinin ; deuterium labelling ; biosynthetic rate
Subject RIV	CE - Biochemistry
R&D Projects	GA522/08/0920 GA ČR - Czech Science Foundation (CSF)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA301/08/1649 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000285709000046
DOI	10.3390/molecules15129214
Annotation	A set of 11 relevant isoprenoid cytokinins, including zeatin isomers, was separated by ultra performance liquid chromatography in less than 6 min. The iP-type cytokinins were observed to give rise to a previously-unknown fragment at m/z 69; we suggest that the diagnostic (204-69) transition can be used to monitor the biosynthetic rate of isopentenyladenine. Furthermore, we found that by treating the cytokinin nucleotides with alkaline phosphatase prior to analysis, the sensitivity of the detection process could be increased. In addition, derivatization (propionylation) improved the ESIMS response by increasing the analytes' hydrophobicity. Indeed, the ESI-MS response of propionylated isopentenyladenosine was about 34% higher than that of its underivatized counterpart. Moreover, the response of the derivatized zeatin ribosides was about 75% higher than that of underivatized zeatin ribosides. Finally, we created a web-based calculator (IZOTOP) that facilitates MS/MS data processing.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1