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Endogenous cytokinins in Cocos nucifera L. in vitro cultures obtained from plumular explants

SYSNO ASEP	0359284
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Endogenous cytokinins in Cocos nucifera L. in vitro cultures obtained from plumular explants
Author(s)	Sáenz, L. (MX) Azpeitia, A. (MX) Oropeza, C. (MX) Jones, L. (GB) Fuchsová, Květoslava (UEB-Q) Spíchal, Lukáš (UEB-Q)_{RID, ORCID} Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Number of authors	7
Source Title	Plant Cell Reports. - : Springer - ISSN 0721-7714 Roč. 29, č. 11 (2010), s. 1227-1234
Number of pages	8 s.
Language	eng - English
Country	DE - Germany
Keywords	Coconut palm ; Somatic embryogenesis ; Endogenous cytokinins
Subject RIV	EF - Botanics
R&D Projects	GA301/08/1649 GA ČR - Czech Science Foundation (CSF)
	IBS5038351 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000283096000003
DOI	10.1007/s00299-010-0906-9
Annotation	Embryogenic calli and non-embryogenic calli can be formed from the initial calli. Fourteen cytokinins were analysed in the three types of calli and all the cytokinins were found in each type. The most abundant cytokinins in each type of callus were isopentenyladenine-9-glucoside, zeatin-9-glucoside, zeatin riboside, isopentenyladenine riboside, dihydrozeatin and dihydrozeatin riboside in decreasing order. Total cytokinin content was found to be lower in embryogenic calli compared to non-embryogenic calli or initial calli. When explants were cultured in media containing exogenously added cytokinins, the formation of embryogenic calli in the explants was reduced. When 8-azaadenine (an anticytokinin) was added the formation of embryogenic calli and static embryos was increased. These results suggest that the difference in somatic embryo formation capacity observed between embryogenic calli and non-embryogenic calli is related to their endogenous cytokinin contents.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1