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PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca

SYSNO ASEP	0359234
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca
Author(s)	Lajbner, Zdeněk (UZFG-Y)_RID Kotlík, Petr (UZFG-Y)_{RID, ORCID}
Source Title	Molecular Ecology Resources - ISSN 1755-098X Roč. 11, č. 2 (2011), s. 374-377
Number of pages	4 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	EPIC ; exon-primed intron-crossing marker ; mtDNA
Subject RIV	EG - Zoology
R&D Projects	LC06073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000287200200019
DOI	10.1111/j.1755-0998.2010.02914.x
Annotation	The tench Tinca tinca is a valued table fish which is being transplanted between watersheds without concern for genetic similarity to wild populations, and efficient phylogeographic markers were therefore urgently needed to rapidly distinguish genetically distinct geographic populations and to assess their contribution to the hatchery breeds and to the stocked wild populations. The new method developed in this study relies on PCR-RFLP assays of two independent nuclear-encoded markers and of one mitochondrial DNA marker, and allows the rapid identification of the Western and Eastern phylogroup and also of three geographical mtDNA clades within the Eastern phylogroup. The method will be useful for monitoring the introduction and human-mediated spread of the phylogroups in wild populations, for characterisation of cultured strains and in breeding experiments.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2012

Number of the records: 1